Momwe mungasankhire zinthu zogwiritsidwa ntchito pa PCR/qPCR?

PCR ndi njira yachidziwitso komanso yothandiza yokulitsa kopi imodzi ya DNA yomwe mukufuna kukhala mamiliyoni ambiri munthawi yochepa. Chifukwa chake, zopangira pulasitiki pazochita za PCR ziyenera kukhala zopanda zowononga ndi zoletsa, pomwe zili ndi mawonekedwe apamwamba omwe angatsimikizire zotsatira zabwino kwambiri za PCR. Zogwiritsira ntchito pulasitiki za PCR zimapezeka m'makulidwe ndi maonekedwe osiyanasiyana, ndipo kudziwa makhalidwe oyenera azinthuzo kudzakuthandizani kusankha zogwiritsira ntchito pulasitiki zoyenera PCR ndi qPCR deta.

Mapangidwe ndi Makhalidwe a PCR consumables

1.Zinthu
Zomwe zimagwiritsidwa ntchito pa PCR nthawi zambiri zimapangidwa ndi polypropylene, yomwe imakhala yokwanira kupirira kutentha kwachangu pakapita njinga yotentha ndikuchepetsa kutengeka kwa zinthu zowonongeka kuti zitsimikizire zotsatira zabwino za PCR. zachipatala, zida zapamwamba za polypropylene ziyenera kugwiritsidwa ntchito popanga ndikupangidwa mchipinda choyera cha Gulu 100,000. Chogulitsacho chiyenera kukhala chopanda ma nuclease ndi DNA kuipitsidwa kuti zisasokoneze zotsatira za kuyesa kukulitsa kwa DNA.

2. Mtundu
Zithunzi za PCRndiMachubu a PCRnthawi zambiri amapezeka mu transparent and white.

• The yunifolomu khoma makulidwe kapangidwe adzapereka mogwirizana kutentha kusamutsa kwa anachita zitsanzo.

• High kuwala permeability kuonetsetsa mulingo woyenera fluorescence siginecha kufala ndi zochepa kupotoza.

• M'mayesero a qPCR, dzenje loyera limalepheretsa kusinthika kwa chizindikiro cha fluorescence ndi kuyamwa kwake ndi gawo lotenthetsera.

3. Mtundu
Chithunzi cha PCR "skirt" ili kuzungulira bolodi. Siketiyo imapereka kukhazikika bwino kwa njira yopangira mapaipi pomwe njira yochitira imamangidwa, komanso imapereka mphamvu zamakina bwino panthawi yamankhwala odzipangira okha. PCR mbale akhoza kugawidwa popanda siketi, siketi theka ndi siketi zonse.

• Mbale ya PCR yopanda siketi ikusowa pozungulira mbaleyo, ndipo mawonekedwe amtundu uwu amatha kusinthidwa pazida zambiri za PCR ndi ma module a zida za PCR zenizeni, koma osati zongogwiritsa ntchito zokha.

• Mbalame ya PCR ya semi-skirt ili ndi m'mphepete mwaifupi kuzungulira m'mphepete mwa mbale, kupereka chithandizo chokwanira panthawi ya pipetting ndi mphamvu zamakina zogwirira ntchito robotic.

• Mbale ya PCR yokhala ndi siketi yonse ili ndi m'mphepete mwake yomwe imaphimba kutalika kwa mbale. Fomu ya mbale iyi ndi yoyenera kuzigwiritsa ntchito zokha, zomwe zingakhale zotetezeka komanso zokhazikika. Siketi yathunthu imapangitsanso mphamvu zamakina, ndikupangitsa kuti ikhale yabwino kugwiritsidwa ntchito ndi ma robot pamayendedwe odzichitira okha.

PCR chubu imapezeka mu chubu limodzi ndi 8-strips chubu, yomwe ili yoyenera kwambiri pakuyesa kwapakatikati kapena kocheperako kwa PCR/qPCR. Chophimba chathyathyathya chidapangidwa kuti chithandizire kulemba, ndipo kufalikira kwakukulu kwa siginecha ya fluorescence kumatha kuzindikirika bwino ndi qPCR.

• The single chubu amapereka kusinthasintha kukhazikitsa chiwerengero chenicheni cha zochita. Kwa ma voliyumu okulirapo, chubu limodzi la kukula kwa 0.5 mL likupezeka.
• Machubu a 8-strips okhala ndi zipewa amatsegula ndikutseka machubu achitsanzo mopanda kuti aletse zitsanzo.

4.kusindikiza
Chophimba cha chubu ndi filimu yosindikizira iyenera kusindikiza chubu ndi mbale kuti zisawonongeke kuti zisamawonongeke panthawi ya kutentha. Kusindikiza kolimba kumatha kuzindikirika pogwiritsa ntchito scraper ya filimu ndi chida chosindikizira.

• Zitsime za mbale za PCR zili ndi m'mphepete mwake mozungulira. Kapangidwe kameneka kamathandizira kusindikiza mbale ndi filimu yosindikizira kuti zisawonongeke.
• Zizindikiro za alphanumeric pa PCR mbale zithandizira kuzindikira zitsime zapayekha komanso malo a zitsanzo zofananira. Zilembo zopindika nthawi zambiri zimasindikizidwa zoyera kapena zakuda, ndipo pazogwiritsa ntchito zokha, zilembo zimapindulitsa kwambiri kusindikiza m'mphepete mwa mbale.

5.Flux ntchito

Kuyesa kwa kuyesa kwa PCR / qPCR kutha kudziwa kuti ndi mtundu wanji wazinthu zapulasitiki zomwe ziyenera kugwiritsidwa ntchito kuti zithandizire bwino kwambiri. Pazogwiritsa ntchito zocheperako, machubu nthawi zambiri amakhala oyenera, pomwe mbale ndizofunika kwambiri pakuyesa kwapakati mpaka kumtunda. Ma mbale amapangidwanso kuti aganizire kusinthasintha kwa flux, komwe kumatha kugawidwa mumzere umodzi.

Pomaliza, monga gawo lofunikira pakumanga kwadongosolo la PCR, zogwiritsira ntchito pulasitiki ndizofunikira kuti zoyeserera zitheke komanso kusonkhanitsa deta, makamaka pamapulogalamu apakatikati mpaka apamwamba.

Monga ogulitsa aku China opangira pulasitiki, Cotaus amapereka malangizo a pipette, nucleic acid, kusanthula mapuloteni, chikhalidwe cha maselo, kusungirako zitsanzo, kusindikiza, chromatography, etc.

Dinani pamutu wazinthu kuti muwone zambiri zazinthu za PCR consumables.

PCR Tube ;Chithunzi cha PCR