Kodi abwenzi omwe ali mu labotale nthawi zambiri amasokonezeka chifukwa cha kusiyana kwa machubu a PCR, ma EP, ndi machubu asanu ndi atatu? Lero ndikuwonetsa kusiyana ndi mawonekedwe a atatuwa

Kodi mabwenzi mu labotale nthawi zambiri kusokonezeka ndi kusiyana pakatiPCR chubus, machubu a EP, ndi machubu asanu ndi atatu? Lero ndikuwonetsa kusiyana ndi mawonekedwe a atatuwa

1. PCR chubu

PCR chubus ndi zinthu zomwe zimagwiritsidwa ntchito kwambiri pakuyesa kwachilengedwe. Mwachitsanzo, machubu a Cotaus®PCR amagwiritsidwa ntchito makamaka popereka zotengera zoyeserera za PCR (polymerase chain reaction), zomwe zitha kugwiritsidwa ntchito pakusintha, kutsatizana, methylation, cloning molecular, gene expression, Genotyping, medicine, forensic science ndi zina. Chingwe chodziwika bwino cha PCR chimapangidwa ndi thupi la chubu ndi chivundikiro, ndipo thupi la chubu ndi chivundikiro zimalumikizidwa palimodzi.

Chida choyambirira cha PCR chinalibe chivundikiro chotentha. Panthawi ya PCR, madzi omwe ali pansi pa chubu amatha kusanduka nthunzi pamwamba. Chivundikiro cha convex (ndiko kuti, chozungulira pamwamba) chidapangidwa kuti chithandizire kutuluka kwamadzimadzi kuti nthike ndikutsika pansi. Komabe, chida chamakono cha PCR kwenikweni ndi mtundu wa chivundikiro chotentha. Kutentha pamwamba pa chivundikiro cha PCR ndipamwamba ndipo kutentha pansi kumakhala kochepa. Madzi omwe ali pansi siwophweka kuti asungunuke pamwamba, kotero ambiri a iwo amagwiritsa ntchito zophimba zathyathyathya.

2. EP chubu

Chifukwa chubu cha centrifuge chinapangidwa koyamba ndikupangidwa ndi Eppendorf, chimatchedwanso EP chubu.

Kusiyana kwakukulu pakatiPCR chubus ndi machubu a microcentrifuge ndikuti machubu a microcentrifuge nthawi zambiri amakhala ndi makoma okulirapo kuti atsimikizire zofunikira za centrifugation, pomwePCR chubus ali ndi machubu ocheperako kuti awonetsetse kuthamanga kwa kutentha komanso kufanana. Choncho, ziwirizi sizingasakanizidwe muzogwiritsira ntchito, chifukwa machubu ocheperako a PCR amatha kuphulika chifukwa cholephera kupirira mphamvu zazikulu za centrifugal; chimodzimodzi, thicker microcentrifuge machubu adzakhudza zotsatira za PCR chifukwa chapang'onopang'ono kutentha kutengerapo ndi kusamutsa kutentha mosiyanasiyana.

3.machubu asanu ndi atatu

Chifukwa cha kuchuluka kwa ntchito pakuyesa kwa batch komanso kusagwira bwino ntchito kwa chubu limodzi, machubu asanu ndi atatu m'mizere adapangidwa.Cotaus®PCR 8-strip chubu imapangidwa ndi polypropylene yochokera kunja, ndipo chivundikiro cha chubu chimafanana ndi thupi la chubu, chomwe chimakhala ndi ntchito yabwino yosindikiza. Panthawi imodzimodziyo, imakhala ndi mphamvu yosinthika ndipo imatha kukwaniritsa zolinga zosiyanasiyana zoyesera.