Kufika Kwatsopano | ZOKHUDZA | Black Elisa Plates

Muzochitika zosiyanasiyana mu sayansi ya moyo, kutsimikiza kwanthawi yake, kothandiza komanso kwachuma komanso kuchuluka kwa ma antigen kapena ma antibodies omwe amapezeka mu zitsanzo ndi gawo lofunikira.

Ma enzyme olumikizidwa ndi ma immunosorbent assay (ELISA) awonetsa kuti ndi chida chamtengo wapatali chofufuzira komanso chida chowunikira pakuyeza ma antibodies kapena ma antigen mu zitsanzo zachilengedwe potengera ma antigen odziwika kapena ma antibodies pamwamba pa chonyamulira cholimba, chomwe chimalola enzyme. makamaka HRP) -otchedwa antigen-antibody reactions pa olimba-gawo pamwamba. Njirayi ingagwiritsidwe ntchito kuzindikira ma antigen akuluakulu a molekyulu ndi ma antibodies enieni, etc. Ili ndi ubwino wokhala wofulumira, womvera, wosavuta, ndipo chonyamuliracho ndi chosavuta kulinganiza. Komabe, kukhudzika ndi kusinthika kwamtundu wa ELISA kuzindikira kumakhala kochepa kwambiri ndi zofooka za njira yoyamwitsa kuwala chifukwa cha mphamvu yaikulu ya zinthu zakunja pa kusintha kwa mtundu wa yankho ndi kutsika kothandiza kwa mzere wamtengo wapatali wa OD.

Tekinoloje ya DELFIA ---- ndikungolowetsa enzyme ya HRP ndi lanthanide chelate (Eu, Sm, Tb, Dy) yolemba pa antibody yodziwikiratu muzoyesa zachikhalidwe za ELISA. Ma lanthanides omwe amagwiritsidwa ntchito ku DELFIA ndi gulu lapadera la zinthu za fulorosenti, zomwe zimayika zofunikira pazoyesera --- mbale za Elisa. Lanthanides ali ndi nthawi ya fluorescence ya ma microseconds kapena ma milliseconds, omwe kuphatikiza ndi kuzindikira kwanthawi yayitali amachepetsa kwambiri kusokoneza kwa autofluorescence, ndipo kusintha kwawo kwa Strokes kumathandizira kwambiri chidwi cha kuyesa.

Ambiri a ELISA amasankha mbale yolembera ma enzyme yowonekera ngati chonyamulira ndi chidebe, koma kuwala komwe kumatulutsa mumayendedwe a luminescence ndi isotropic, kuwala sikungomwazikana kuchokera kunjira yoyima, komanso kumwazikana kuchokera kunjira yopingasa, ndipo kudzatero. mosavuta kudutsa kusiyana pakati pa mabowo osiyanasiyana a mandala enzyme kulemba mbale ndi khoma la holes.Neighboring mabowo kucheza ndi mzake ndi kukhudza zotsatira experimental.

White Elisa Plates zitha kugwiritsidwa ntchito pozindikira kuwala kofooka ndipo nthawi zambiri amagwiritsidwa ntchito ngati chemiluminescence komanso kakulidwe kamtundu wa gawo lapansi (monga dual luciferase reporter gene analysis).

Black White Elisa Plates ali ndi chizindikiro chofooka kuposa mbale zoyera za enzyme chifukwa cha kuyamwa kwawo kwa kuwala, ndipo nthawi zambiri amagwiritsidwa ntchito pozindikira kuwala kwamphamvu, monga kuzindikira kwa fluorescence.

Ubwino wa Cotaus®Elisa Plates

● Kumanga kwakukulu

Ma mbale a Cotaus®Elisa okhala ndi chubu chakuda amapangidwa ndi zinthu zopanda fulorosenti, pamwamba pake adathandizidwa kuti apititse patsogolo mphamvu yake yomanga mapuloteni, yomwe imatha kufika 500ng IgG/cm2, ndipo kulemera kwa mamolekyu a mapuloteni akuluakulu ndi> 10kD. .

● Kutsika kwa fluorescence kumathetsa mavuto omwe amadza chifukwa cha kusagwirizana kwachindunji.

machubu akuda amatha kuthetsa kusokoneza kwamtundu wina wofooka wa fluorescence chifukwa adzakhala ndi mayamwidwe akeake.

● Kapangidwe kake

Mapangidwe osinthika a chimango cha mbale yoyera ya enzyme ndi ma slats akuda a enzyme ndiwosavuta kugwira ntchito. Samalani ndi disassembling kanthu, musakakamize kuswa pa mapeto amodzi, mwinamwake kudzakhala kosavuta kuswa.

Gulu lazinthu

Kuti mudziwe zambiri zamalonda, chonde omasuka kulankhula nafe