Chiyambi cha Elisa Plate

ELISA PLATE: Mu Enzyme Linked Immunosorbent Assay (ELISA), chiyero, ndende ndi chiŵerengero cha ma antigen, ma antibodies, ma antibodies olembedwa kapena ma antigen omwe akukhudzidwa ndi machitidwe a immunological; mtundu wa buffer, ndende ndi Mikhalidwe monga mphamvu ya ayoni, mtengo wa pH, kutentha kwamachitidwe ndi nthawi zimatenga gawo lalikulu. Kuonjezera apo, pamwamba pa polystyrene yolimba-phase (Polystyrene) monga chonyamulira imakhalanso ndi gawo lofunika kwambiri pakupanga ma antigen, ma antibodies kapena antigen-antibody complexes.

Antigens, ma antibodies ndi ma biomolecules ena amadsorbed kwa chonyamulira pamwamba kudzera njira zosiyanasiyana, kuphatikizapo kungokhala chete adsorption kudzera hydrophobic zomangira, hydrophobic/ionic zomangira, covalent kugwirizana kudzera kumayambiriro kwa magulu ena yogwira monga magulu amino ndi mpweya, ndi kupyolera pamwamba kusinthidwa. . Kugwirizana kwa Hydrophilic pambuyo pa kugonana.

TheElisa Plateakhoza kugawidwa mu 48-chitsime ndi 96-chabwino malinga ndi kuchuluka kwa mabowo. Zomwe zimagwiritsidwa ntchito kwambiri ndi 96-chabwino, zomwe ziyenera kusankhidwa malinga ndi owerenga anu a microplate.

Kuphatikiza apo, pali zotayika komanso zosasunthika. Kwa omwe sangawonongeke, ma slats pa bolodi lonse amagwirizanitsidwa palimodzi. Ndiye, kwa omwe amatha kuchotsedwa, ma slats pa bolodi amasiyanitsidwa, ndipo matabwa olekanitsidwa Pali 12-hole ndi 8-bowo mizere. Nthawi zambiri, mbale zopezeka ndi ma enzyme zimagwiritsidwa ntchito masiku ano. Ngati mudagulapo mbale zotere, mutha kungogula zingwe zolembedwa ma enzyme tsopano.

Ngakhale ma microplates opangidwa ndi opanga osiyanasiyana amawoneka ofanana onse, zina zing'onozing'ono zidzakhala zosiyana, monga mapangidwe, ndi zina zotero. Izi makamaka chifukwa ziyenera kugwiritsidwa ntchito ndi owerenga ma microplate osiyanasiyana. Chifukwa chake, mukamagwiritsa ntchito Posankha kugula chowerengera cha microplate, muyenera kuganiziranso momwe owerengera anu a microplate amawonekera. Koma kawirikawiri amasinthidwa, ena okha ndi omwe angakhale osiyana. Chifukwa zinthu za mbale ya enzyme nthawi zambiri zimakhala polystyrene (PS), ndipo polystyrene imakhala yosakhazikika bwino pamankhwala ndipo imatha kusungunuka ndi zosungunulira zosiyanasiyana za organic (monga ma hydrocarbon onunkhira, ma halogenated hydrocarbons, ndi zina zotero), ndipo zidzapangitsidwa ndi ma acid amphamvu. ndi alkalis. , osagonjetsedwa ndi mafuta, komanso osavuta kusintha mtundu pambuyo poyang'aniridwa ndi kuwala kwa ultraviolet, choncho onetsetsani kuti mukutsatira izi mukamagwiritsa ntchitoElisa Plate.