Chifukwa chiyani ma PCR nthawi zambiri amapangidwa ndi PP?

"Monga tonse tikudziwa, PCR ndi njira yoyesera yoyambira m'ma laboratories a biochemical." Zotsatira zoyesera nthawi zonse zimakhala zosakhutiritsa, zomwe zingakhale chifukwa cha kuipitsidwa pang'ono kwa PCR pulasitiki consumables, kapena kusokoneza kuyesera chifukwa cha kuyambitsa zoletsa. Palinso chifukwa china chofunika kwambiri: Kusankhidwa kosayenera kwa zogwiritsira ntchito kudzakhalanso ndi zotsatira zoyesa.

Pali zifukwa zambiri zomwe zimakhudza zotsatira za kuyesa kwa PCR: nthawi zambiri pamakhala mitundu 7 yotsatirayi.

1. Zoyambira: Zoyambira ndizofunika kwambiri pakuchitapo kanthu kwa PCR, ndipo kutsimikizika kwazinthu za PCR kumadalira kuchuluka kwa kuyanjana pakati pa zoyambira ndi template ya DNA;

2. Enzyme ndi ndende yake;

3. Ubwino ndi kuchuluka kwa dNTP;

4. Template (chandamale jini) nucleic acid;

5. Kukhazikika kwa Mg2 +;

6. Kuyika kwa kutentha ndi nthawi;

7. Chiwerengero cha zozungulira;

8. Zida, zogwiritsira ntchito, etc.

Pakati pa zinthu zambiri zomwe zimakhudza, zogwiritsidwa ntchito ndi chimodzi mwazinthu zofunika kwambiri komanso zosavuta kuzinyalanyaza.

Pali mitundu yambiri yaZithunzi za PCR: 8-machubu, machubu otsika kwambiri, machubu okhazikika, osavala masiketi, ovala semi-skirts, odzaza ndi mapepala a PCR ndi qPCR. Ndizovuta kusankha, ndipo pali mavuto ambiri Ambiri, tiyeni tione mavuto amene aliyense amasankhaZithunzi za PCR, ndi momwe angawathetsere?

Chifukwa chiyaniZithunzi za PCRzambiri zopangidwa ndi PP?

Yankho: PCR/qPCR consumables zambiri zopangidwa polypropylene (PP), chifukwa ndi zinthu biologically inert, pamwamba si zophweka kutsatira biomolecules, ndipo ali wabwino kukana mankhwala ndi kutentha kulolerana (akhoza autoclaved pa madigiri 121) mabakiteriya. komanso imatha kupirira kusintha kwa kutentha panthawi yanjinga yotentha). Zidazi nthawi zambiri zimalumikizana mwachindunji ndi ma reagents kapena zitsanzo, kotero zida zapamwamba komanso njira zabwino zogwirira ntchito ziyenera kusankhidwa panthawi yopanga ndikukonzekera.