Kugwiritsa Ntchito Wamba kwa ELISA Plates

Monga chida choyesera, mawonekedwe apakati aELISA mbalendi ma microplates angapo okhala ndi zida zolimba (monga mapuloteni ndi ma antibodies). Pogwiritsa ntchito mbale ya ELISA, chitsanzo choyesedwa chidzachitapo kanthu ndi molekyulu yodziwika bwino ya enzyme, kenako kusintha kwamtundu wowoneka kudzapangidwa powonjezera gawo lapansi la matrix, ndipo zomwe zili kapena ntchito ya molekyu yomwe mukufuna idzawerengedwa. kapena kuwunikiridwa pozindikira chizindikiro cha kuyamwa kapena fulorosenti. Zotsatirazi ndizomwe zimagwiritsidwa ntchito kwambiri ndi mbale za ELISA m'magawo osiyanasiyana:

1. Kusanthula kachulukidwe ka mapuloteni: mbale za ELISA zitha kugwiritsidwa ntchito kuyeza kuchuluka kwa mapuloteni ndi ntchito zamapuloteni mu zitsanzo zachilengedwe monga seramu ndi ma cell supernatants, kupereka zida zamphamvu zodziwira zolembera zotupa, ma antibodies a hepatitis virus, zolembera zovulala za myocardial, ndi zina zambiri. ndi kuthandiza madokotala kuti adziwe matenda oyambirira ndi kuyezetsa matenda.

2. Kuwunika kwa Cytokine: Mu kafukufuku wa immunology,ELISA mbaleimatha kuyeza kuchuluka kwa ma cytokine m'ma cell culture supernatants kapena madzi amadzimadzi, omwe amathandiza kumvetsetsa njira zachilengedwe monga mayankho a chitetezo chamthupi ndi mayankho otupa, ndipo ndizofunikira kwambiri pakupanga mankhwala atsopano ndi mankhwala.

3. Kafukufuku wa Nucleic acid: Kupyolera mu mbale za ELISA, asayansi amatha kuzindikira ndi kusanthula zomwe zili ndi ntchito za DNA kapena RNA, kupereka chithandizo cha data pa kafukufuku wa sayansi ya maselo monga gene expression ndi gene regulation, ndikulimbikitsanso chitukuko cha madera monga gene therapy. ndi kusintha gene.

4. Kafukufuku wa ntchito ya enzyme: mbale za ELISA zimatha kuyeza molondola ntchito ya enzyme, kuthandizira ofufuza kumvetsetsa momwe ma enzyme amagwirira ntchito m'zamoyo, ndikupereka maumboni ofunikira pa kafukufuku wa enzyme engineering, metabolic engineering ndi zina.

5. Kafukufuku wokhudzana ndi ma cell:ELISA mbaleangagwiritsidwe ntchito osati kuyeza zomwe zili mu mamolekyu, komanso kuphunzira kugwirizana pakati pa mamolekyu. Mwa kuphatikiza matekinoloje monga pamwamba pa plasmon resonance ndi fluorescence resonance resonance mphamvu, njira yomangiriza ndi kupatukana pakati pa mamolekyu imatha kuyang'aniridwa mu nthawi yeniyeni, kupereka malingaliro atsopano ndi njira zopangira mankhwala, kuyanjana kwa mapuloteni ndi kafukufuku wina.